Research
The main objective of our lab is to understand epigenetic mechanisms underlying host-parasite interactions. We are particularly interested in neglected tropical parasites.
Our research focuses on understanding how small RNAs regulate the parasite's lifecycle and pathogenesis and influence host-pathogen interaction. Also, our research will focus on understanding the biogenesis of the parasite small RNAs by atypical parasite small RNA machinery and the influence of stress and host factors on small RNA production and their function.
The three main questions include
1. How small RNAs are synthesized?
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2. What is the role of the small RNA pathway in the life cycle of parasites?
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3. How do small RNAs regulate host-pathogen interaction?
Icons from Biorender
We are using state-of-the-art tools which we are constantly evolving based on our questions and requirements. These include:
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small RNA and mRNA sequencing (Singh et al 2021a, b)
measurement of Translation by Ribo-seq (Singh et al 2021a)
Global Run on Sequencing ( to measure transcription) (Singh et al 2021a, Barucci et al 2020)
individual-nucleotide resolution Cross-Linking and ImmunoPrecipitation & sequencing (iCLIP-seq)
Proteomics, biochemical and molecular tools to analyze protein-protein and RNA-protein interactions